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NEET · Biology · Class 12

Biotechnology and Its Applications — NEET Biology MCQs

30 questions written by hand against the NCERT chapter. Every wrong option is explained, not just the right one.

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Sample questions from this chapter

The ability of a plant cell or explant to regenerate a complete plant is called
  1. totipotency ✓
  2. transduction
  3. translation
  4. biopiracy
Answer: A. Plant tissue culture exploits totipotency: under sterile conditions and suitable nutrition and growth regulators, an explant can form a whole plant.
Why not B: Transduction is gene transfer mediated by a virus.
Why not C: Translation produces a polypeptide from mRNA.
Why not D: Biopiracy concerns unauthorised commercial use of biological resources or knowledge.
Why must a tissue-culture medium usually contain sucrose along with salts, vitamins and growth regulators?
  1. Sucrose digests the plant wall
  2. Sucrose selects antibiotic-resistant cells
  3. The isolated tissue needs an external carbon and energy source under culture conditions ✓
  4. Sucrose converts a protoplast into a virus
Answer: C. An explant in vitro may not photosynthesise sufficiently. Sucrose supplies carbon, while minerals, vitamins, amino acids, auxins and cytokinins support growth and differentiation.
Why not A: Cell-wall digestion uses enzymes such as cellulase.
Why not B: Antibiotic selection relies on a resistance marker.
Why not D: Sugar nutrition cannot convert a plant cell into a virus.
A Bt cotton plant kills bollworm larvae but a beetle species survives. Which interpretation best follows from Cry biology?
  1. Cry proteins are target-group specific, so the expressed toxin may not bind or act effectively in that beetle gut ✓
  2. Bt toxins act on lepidopteran larvae alone, so beetles are inherently outside their target range
  3. Cotton cells express the toxin solely after insect death
  4. Survival proves that the bacterium never produced a protoxin
Answer: A. Cry toxicity depends on activation and compatible receptors in susceptible midgut cells. Pest specificity explains why a gene effective against one group may spare another.
Why not B: Different Cry proteins target different insect groups, including some coleopterans; specificity belongs to the particular Cry protein, not to Bt toxins as a lepidopteran-only class.
Why not C: The protection requires toxin expression before and during feeding.
Why not D: One non-susceptible insect does not negate Bt production or activity against the target.

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Every NCERT question in this chapter is solved, free: NCERT solutions — Biotechnology and Its Applications →

Other NEET Biology chapters

The Living WorldBiological ClassificationPlant KingdomAnimal KingdomMorphology of Flowering PlantsAnatomy of Flowering Plants

All 32 chapters →

Questions

How many NEET questions are there on Biotechnology and Its Applications?

This chapter test has 30 questions — 10 easy, 14 medium and 6 hard — all written against the NCERT Class 12 chapter.

Is this NEET Biology chapter test free?

Yes. Every chapter test is free with no login, and you get your all-India rank on every one. Nothing on the site is on sale right now.

Do the questions explain the wrong options?

Yes — every distractor carries its own explanation naming the specific misconception that makes a student pick it. That is the part most question banks skip, and it is the part that changes your next attempt.